Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.
Janus Green B chloride
|Melting Point:160ºC (dec.)(lit.)|
|Storage temp:Keep container closed when not in use. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.|
|Vapour:2.6 mm Hg ( 20 °C)|
|Stability:Stable. Incompatible with strong oxidizing agents.|
Safety and Handling